Immunohistochemical Study of O-GlcNAcylation in Human Skin Tumors.
10.11637/kjpa.2014.27.2.71
- Author:
Young LEE
1
;
Dong Kyun HONG
;
Dae Kyoung CHOI
;
Seul Ki LIM
;
Kyung Cheol SOHN
;
Myung IM
;
Young Joon SEO
;
Young Ho LEE
;
Jeung Hoon LEE
;
Chang Deok KIM
Author Information
1. Department of Dermatology and Research Institute for Medical Sciences, School of Medicine, Chungnam National University, Daejeon, Korea. cdkimd@cnu.ac.kr
- Publication Type:Original Article
- Keywords:
Immunohistochemistry;
O-GlcNAc;
O-GlcNAcase;
O-GlcNAc transferase;
Skin neoplasm
- MeSH:
Breast;
Colonic Neoplasms;
Humans;
Immunohistochemistry;
Lung;
Protein Processing, Post-Translational;
Skin Neoplasms;
Skin*;
Transferases
- From:Korean Journal of Physical Anthropology
2014;27(2):71-77
- CountryRepublic of Korea
- Language:English
-
Abstract:
O-linked beta-N-acetylglucosamine modification is an important post-translational modification, emerging as a novel regulatory mechanism in various cellular events. Recently, several studies have shown that O-GlcNAcylation plays an essential role in human breast, lung, and colon cancers. With regard to skin cancers, the role of O-GlcNAcylation has yet to be elucidated. To investigate whether O-GlcNAcylation is linked to human skin tumor development, immunohistochemical analysis was performed to investigate the presence of O-GlcNAcylation in various skin tumors. We evaluated the levels of O-GlcNAcylation, O-GlcNAc transferase, and O-GlcNAcase in 29 benign tumors, 12 premalignant tumors, and 26 malignant tumors in skin. Compared to the benign tumors, premalignant and malignant tumors had increased patterns of O-GlcNAcylation. In addition, the O-GlcNAc transferase and O-GlcNAcase levels were higher in premalignant and malignant tumors than in benign tumors. Interestingly, O-GlcNAcase levels were significantly increased in premalignant tumors compared to benign and malignant tumors. These results suggest that O-GlcNAcylation of proteins may play an important role in the development of human skin tumors.
