Role of Jiawei Shenqi-wan in Treatment of Cathartic Colon on the Basis of Interstitial Cells of Cajal
10.3969/j.issn.1008-7125.2021.01.003
- Author:
Zhenmin ZHU
1
;
Meixue JI
2
;
Pan TANG
2
;
Junliang CHEN
3
Author Information
1. Department of Traditional Chinese Medicine, Second Affiliated Hospital of Yunnan University of Chinese Medicine
2. Graduate Department of Yunnan University of Chinese Medicine
3. Department of Proctology, Yunnan Provincial Hospital of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Cathartic Colon;
Interstitial Cells of Cajal;
Jiawei Shenqi-Wan;
SCF/c-kit
- From:
Chinese Journal of Gastroenterology
2021;26(1):17-23
- CountryChina
- Language:Chinese
-
Abstract:
Background: Cathartic colon belongs to the category of 'constipation' in Traditional Chinese Medicine, and its pathogenesis is related to deficiency of kidney temperament and weak promotion ability, which has become a hot spot of global medical attention. Aims: To investigate the effect and possible mechanism of Jiawei Shenqi-wan (JSQW) on intestinal transmission function and pathological changes of interstitial cells of Cajal (ICCs) in rats with cathartic colon. Methods: Forty rats were randomly divided into blank group, model group, prucalopride group and JSQW group. Fecal moisture content, fecal particle number and intestinal transit rate were detected. The pathological changes of ICCs were observed under transmission electron microscope. RT-qPCR was used to detect the mRNA expressions of water channel proteins (AQP3 and AQP9) and SCF/c-kit pathway. Immunohistochemistry was used to measure the expressions of α-smooth muscle actin (α-SMA), inducible nitric oxide synthase (iNOS) and 5-hydroxytryptamine (5-HT)
