Protective Effect of Ang(1-7) on Small Intestinal Injury in NSAIDs-induced Enteropathy by Regulating Treg/Th17 Balance
10.3969/j.issn.1008-7125.2021.07.003
- Author:
Yijiu PAN
1
;
Liankui WANG
1
;
Hua XIONG
1
;
Hua XIONG
2
Author Information
1. Department of Gastroenterology, Ningbo Hangzhou Bay Hospital
2. Division of Gastroenterology and Hepatology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Institute of Digestive Disease
- Publication Type:Journal Article
- Keywords:
Angiotensin 1-7;
Anti-Inflammatory Agents, Non-Steroidal;
Small Intestinal Injuries;
T-Lymphocytes, Regulatory;
Th17 Cells
- From:
Chinese Journal of Gastroenterology
2021;26(7):395-401
- CountryChina
- Language:Chinese
-
Abstract:
Background: Nonsteroidal antiinflammatory drugs (NSAIDs) are widely used in clinical practice. As the progress of endoscopic techniques, NSAIDs-induced small intestinal injury is more frequently to be detected, but there is still lack of effective preventive and therapeutic measures. Aims: To explore the role of regulatory T cells (Treg cells)/Th17 cells imbalance in NSAIDs-induced small intestinal injury and the protective effect of angiotensin 1-7[Ang(1-7)]. Methods: Thirty male Sprague-Dawley rats were randomly divided into control group, model group, and Ang(1-7) treatment group; in the latter two groups, diclofenac sodium was used to induce small intestinal injury. On day 5, the rats were sacrificed to obtain small intestinal mucosa. The macro- and microscopic changes of the intestinal mucosa were evaluated; the levels of Ang(1-7), and pro- and antiinflammatory cytokines were detected by ELISA and/or immunohistochemistry; flow cytometry was used to determine the proportions of Treg and Th17 cells in CD4
