Study on Expression of LncRNA MEG3 in Gastric Cancer and its Correlation With Glycolysis
10.3969/j.issn.1008-7125.2022.06.002
- Author:
Yunrong LI
1
;
Hong LI
1
;
Yiqun LI
1
;
Yanmin HAN
1
;
Yalong ZHANG
1
;
Yiyi ZHANG
1
;
Zhang CAO
1
;
Qingyong CHEN
2
Author Information
1. Department of Pathology, Binzhou Medical University Hospital, The First School of Clinical Medicine of Binzhou Medical University
2. Department of Medical Affairs, Binzhou Medical University Hospital, The First School of Clinical Medicine of Binzhou Medical University
- Publication Type:Journal Article
- Keywords:
Glycolysis;
LncRNA MEG3;
Prognosis;
Stomach Neoplasms
- From:
Chinese Journal of Gastroenterology
2022;27(6):328-335
- CountryChina
- Language:Chinese
-
Abstract:
Background: Abnormal glucose metabolism is one of the malignant characteristics of tumors. LncRNA plays an important role in the process of aerobic glycolysis of tumors. Aims: To investigate the expression of LncRNA MEG3 in gastric cancer and its correlation with glycolysis. Methods: RT-qPCR was used to detect the mRNA expression of MEG3 in gastric cancer and paracancerous tissue. Immunohistochemical EnVision method was used to detect the protein expressions of PKM2, LDHA, mTOR, HIF-1α in gastric cancer and paracancerous tissue. Relationship between expressions of above-mentioned indices and clinicopathological features of gastric cancer were analyzed. The correlation between MEG3 and glycolysis level of gastric cancer was analyzed by Spearman correlation analysis, and its possible mechanism was explored. Results: The expression of MEG3 in gastric cancer tissue was significantly lower than that in paracancerous tissue (P< 0.05), and was correlated with lymph node metastasis (P<0.05). The positivity rates of expression of PKM2, LDHA, mTOR and HIF-1α in gastric cancer tissue were significantly higher than those in paracancerous tissue, and were correlated with the depth of tumor invasion, lymph node metastasis and pTNM stage (P<0.05). Spearman correlation analysis showed that the expression of MEG3 was negatively correlated with the expressions of PKM2, LDHA, mTOR and HIF-1α (r=-0.346，r= -0.306，r=-0.389, r=-0.338; P<0.05). The expression of MEG3 in HIF-1α
