miR-216b-5p  Promotes the Migration and Invasion of LN-229 Glioma Cells by Targeting BTN3A2

Dan-dan Zhou; Li-ying Zhang; Li-ping Zhang; Quan Zheng; Jun Bai; Ya-qiong HU; Shi-jun LV; Yu Wang; Qing-jie MU; Chong-gao Yin; Dan-dan Zhou; Yu Wang; Li-ying Zhang; Li-ping Zhang; Quan Zheng; Jun Bai; Ya-qiong HU; Qing-jie MU; Chong-gao Yin; Hong-li LI; Shi-jun LV; Hong-li LI

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miR-216b-5p Promotes the Migration and Invasion of LN-229 Glioma Cells by Targeting BTN3A2

Author: Dan-Dan ZHOU ¹ ; Li-Ying ZHANG ¹ ; Li-Ping ZHANG ¹ ; Quan ZHENG ¹ ; Jun BAI ¹ ; Ya-Qiong HU ¹ ; Shi-Jun LV ¹ ; Yu WANG ² ; Qing-Jie MU ² ; Chong-Gao YIN ³ ; Dan-Dan ZHOU ⁴ ; Yu WANG ⁴ ; Li-Ying ZHANG ⁴ ; Li-Ping ZHANG ⁴ ; Quan ZHENG ⁴ ; Jun BAI ⁴ ; Ya-Qiong HU ⁴ ; Qing-Jie MU ⁴ ; Chong-Gao YIN ⁴ ; Hong-Li LI ⁴ ; Shi-Jun LV ⁴ ; Hong-Li LI ⁵
Author Information

1. Department of Pathology
2. College of Biological Science and Technology
3. College of Clinical Medicine
4. College of Nursing
5. Medical Research Center, Weifang Medical University
Publication Type:Journal Article
Keywords: butyrophilin subfamily 3 member A2(BTN3A2); Glioma; invasion; microRNA-216b-5p (miR-216b-5p ); migration
From: Chinese Journal of Biochemistry and Molecular Biology 2021;37(1):109-117
CountryChina
Language:Chinese
Abstract: Accumulating evidence indicated that microRNAs (miRNA) play an important role in tumor invasion and metastasis by regulating their target genes.However, whether the miRNA-216b-5p(miR-216b-5p ) and their target genes butyrophilin subfamily 3 member A2(BTN3A2) promote glioma invasion and metastasis is unclear.This study aims to study whether miR-216b-5p promoted migration and invasion in glioma cells by negatively regulating BTN3A2.The differential expression analysis of GSE15824 and GSE4290 was analyzed by GEO2R.We found that only BTN3A2 is up-regulated in both GSE15824 and GSE4290 (P<0.05).The gene set enrichment analysis (GSEA) analysis indicated BTN3A2 was related to many cancer-related pathways (P<0.05).The results of survival curves showed that the overall survival of patients with high expression of BTN3A2 decreased significantly (P <0.001).The expression of BTN3A2 was increased with the increase of WHO grade (P<0.05), while the expression of BTN3A2 was increased in 1p/19q uncombined deletion and IDH mutant patients (P<0.001).Western blotting results showed that BTN3A2 was up-regulated in seven glioma tissues and glioma cell lines U87, U251 and LN-229 and downregulated in the miR-216b-5p mimics group; Transwell results showed that transfection with BTN3A2 silencing plasmids(si-BTN3A2) or miR-216b-5p mimics plasmids could inhibit the ability of migration and invasion in LN-229 cells in vitro (P<0.05).The online websites predicted miR-216b-5p as a potential target gene of BTN3A2.The survival curve results show that compared with patients with low expression of miR-216b-5p , the survival rate of patients with high expression was significantly increased (P=0.025).The relative expression of miR-216b-5p was decreased in U87, U251 and LN229 cells was detected by real time quantitative PCR (P<0.05).The results of dual luciferase assay showed that BTN3A2 could bind to miR-216b-5p (P<0.05).Transwell experiment results showed that overexpression of miR-216b-5p can inhibit the migration and invasion ability of LN229 cells (P<0.05).In summary, miR-216b-5p promotes the migration and invasion by targeting BTN3A2 of LN-229 glioma cells.