Overexpression of LncRNA SLC25A25-AS1 Inhibits Proliferation‚ Migration‚ and Invasion of HeLa Cells
10.13865/j.cnki.cjbmb.2021.01.1594
- Author:
Wen-Jie WANG
1
;
Ruo-Xuan NI
1
;
Mei ZHAO
1
;
Chang-Zhi HUANG
1
;
Di WANG
2
;
Sheng-Kai HUANG
2
;
Xiao-Jie SUN
3
;
Ping BAI
4
;
Cheng-Zhi LEI
4
Author Information
1. Department of Etiology and Carcinogenesis, State Key Laboratory of Molecular Oncology, Beijing Key Laboratory for Carcinogenesis and Cancer Prevention, National Cancer Center, National Clinical Research Center for Cancer, Cancer Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College
2. Department of Clinical Laboratory, National Cancer Center, National Clinical Research Center for Cancer, Cancer Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College
3. Department of Biochemistry, Qiqihar Medical University
4. Gynecology Department, National Cancer Center, National Clinical Research Center for Cancer, Cancer Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College
- Publication Type:Journal Article
- Keywords:
cervical cancer;
cervical intraepithelial neoplasia(CIN);
HeLa cells;
long non-coding RNA SLC25A25-AS1
- From:
Chinese Journal of Biochemistry and Molecular Biology
2021;37(3):328-338
- CountryChina
- Language:Chinese
-
Abstract:
Long non-coding RNA SLC25A25-AS1 has a tumor inhibition effect in the development of colorectal cancer. However‚ the mechanism of SLC25A25-AS1 in cervical cancer needs further study. We studied the abnormal expression of SLC25A25-AS1 in the serum of the patients with cervical cancer and the patients with cervical intraepithelial neoplasia (CIN) and explored the mechanism of SLC25A25-AS1 in the development of cervical cancer. The expression levels of SLC25A25-AS1 in the serum of normal controls‚ patients with cervical cancer‚ and patients with CIN were detected by RT-qPCR. The important role of SLC25A25-AS1 in HeLa cells was analyzed in vitro and in vivo experiments. Compared with the normal group‚ the expression level of serum SLC25A25-AS1 was decreased in patients with cervical cancer. In vitro‚ overexpression of SLC25A25-AS1 inhibited cell proliferation‚ migration‚ and invasion of HeLa cells. Tumor formation in nude mice assay showed that the subcutaneous tumor weight and volume of nude mice injected with SLC25A25-AS1-overexpressed HeLa cells were significantly smaller than that of nude mice injected with control cells (P<0. 05). SLC25A25-AS1 may play an important role in the development of cervical cancer and may serve as a new therapeutic target for cervical cancer.
