FNDC5 Regulates the Adipogenic Differentiation of C3H10T1/2 Cells by Inhibiting the Phosphorylation of ERK1/2
10.13865/j.cnki.cjbmb.2021.03.1611
- Author:
Wei HEI
1
;
Zhi-Qiang HE
1
;
Yan-Wei ZHANG
1
;
Wan-Feng ZHANG
1
;
Chun-Bo CAI
1
;
Yang YANG
1
;
Peng-Fei GAO
1
;
Xiao-Hong GUO
1
;
Guo-Qing CAO
1
;
Bu-Gao LI
1
Author Information
1. College of Animal Sciences, Shanxi AgriculturalUniversity
- Publication Type:Journal Article
- Keywords:
adipogenic differentiation;
C3H10T1/2 cells;
extracellular regulated kinase1/2(ERK1/2);
type III domain-containing protein5 (FNDC5)
- From:
Chinese Journal of Biochemistry and Molecular Biology
2021;37(5):644-652
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to explore the regulatory mechanism of Type Ⅲ domain-containing protein5 (FNDC5) on adipogenic differentiation in C3H10T1/2 cells. qRT-PCR and Western blot were used to detect the expression of FNDC5 during adipogenic differentiation of C3H10T1/2 cells. The lentivirus-coated overexpression and interference vector of FNDC5 were constructed and transfected into C3H10T1/2 cells. qRT-PCR was used to detect the expression of the key genes of adipogenic differentiation. Oil red O staining was used to detect the formation of lipid droplets; Western blot was used to detect the content of ERK1/2 and ERK1/2 phosphorylated protein (P-ERK1/2). After 8 days of adipogenic differentiation of C3H10T1/2 cells, the expression of Fndc5 increased significantly. After overexpression of FNDC5 in C3H10T1/2 cells, the expression of key genes for adipogenic differentiation, including peroxisome proliferator-activated receptor-酌 (PPAR酌), CCAAT enhancer binding protein beta (C/EBP茁), fatty acid binding protein 4 (FABP4) and CCAAT enhancer binding protein alpha (C/EBPα), all decreased significantly. The content of lipid droplets and P-ERK1/2 also decreased significantly. On the contrary, after interference of FNDC5 in C3H10T1/2 cells, the expression of key genes for adipogenic differentiation, including PPARγ, C/EBP茁, FABP4 and C/EBPα were significantly increased. Meanwhile, the content of lipid droplets and P-ERK1/2 also increased significantly. This study found that FNDC5 can inhibit the adipogenic differentiation of C3H10T1/2 cells by inhibiting the phosphorylation level of ERK1/2, which can provide reference data for the mechanism of FNDC5 in regulating fat deposition.
