Inhibition of Metformin Combined with Cisplatin on Proliferation of Human Osteosarcoma MG-63 Cells
10.13865/j.cnki.cjbmb.2021.04.1680
- Author:
Xiang ZHANG
1
;
Shuai-Shuai ZHAO
2
;
Ya-Ling WANG
2
;
Jie ZHANG
3
;
Song-Na YIN
4
;
Bo WU
5
Author Information
1. Department of Medical Genetics & Cell Biology, Medical School of Yan’ an University
2. Key Laboratory of Neuroscience in Yan’an, Medical School of Yan’ an University
3. Department of Anatomy, Medical School of Yan’an University
4. Department of Physiology, Medical School of Yan’ an University
5. Department of Histology and Embryology, Medical School of Yan’ an University
- Publication Type:Journal Article
- Keywords:
cisplatin (DPP);
metformin (Met);
osteosarcoma (OS);
proliferation
- From:
Chinese Journal of Biochemistry and Molecular Biology
2021;37(7):960-966
- CountryChina
- Language:Chinese
-
Abstract:
In order to study the effect and its potential mechanism of metformin combined with cisplatin treatment on human osteosarcoma MG-63 cells, MG-63 cells were treated with metformin and cisplatin and the cell proliferation and apoptosis were detected using CCK8 and flow cytometry; Cell clone formation experiment was performed to detect clone formation rate in each group; Trans well experiment was used to detect the migration and invasion ability of osteosarcoma MG-63 cells, and qPCR and Western blot were used to detect the expression of RNA and protein of apoptosis-related genes. The results showed that metformin combined with cisplatin inhibited the proliferation of human osteosarcoma MG-63 cells and promoted their apoptosis significantly (P < 0. 01); Metformin combined with cisplatin inhibited the clone formation (P < 0. 01), and the migration and invasion of human osteosarcoma MG-63 cells (P < 0. 01); Furthermore, metformin combined with cisplatin down-regulated the expression of apoptosis-related genes MCl￣1and XIAP (P <0. 01), but up-regulated the expression of apoptosis-related genes CASPASE￣3 and Cyto C (P <0. 01) and migration and invasion related genes MMP￣2 and MMP￣9 (P <0. 01). Our study indicated that metformin combined with cisplatin inhibited proliferation, promoted cell apoptosis through MCl￣1 and XIAP, and inhibited cell migration and invasion by regulating the MMP￣2 and MMP￣9 pathways in human osteosarcoma MG-63 cells.
