Protective Effects of Raspberry Crude Polysaccharides on UVB-Induced Photodamage in HaCaT Cells
10.13865/j.cnki.cjbmb.2022.08.1061
- Author:
Xing-Xing YIN
1
;
Xue-Hong WANG
1
;
Wen-Hui TIAN
1
;
Yong-Jing YANG
1
;
Mu CHAO
2
Author Information
1. College of Ecology-Environment Engineering, Qinghai University
2. Qinghai Raspberry Agricultural Industrialization Co., Ltd
- Publication Type:Journal Article
- Keywords:
anti-photodamage;
human immortalized keratinocytes (HaCaT);
raspberry crude polysaccharide (RCP)
- From:
Chinese Journal of Biochemistry and Molecular Biology
2022;38(10):1359-1369
- CountryChina
- Language:Chinese
-
Abstract:
Polysaccharide is one of the functional components of the raspberry, which has various pharmacological effects such as anti-inflammatory, antioxidant, anti-fatigue, hypoglycemic and immunomodulatory. However, whether raspberry polysaccharides have protective effects on UV-induced photodamage to skin cells has not been reported. This study aims to investigate the protective effect of Raspberry Crude Polysaccharide on Ultraviolet B (UVB) -induced photodamage of human immortalized keratinocytes (H a C a T). The photodamage model of HaCaT cells was established by UVB irradiation. To evaluate the anti-UVB activity of R C P, the cell viability was determined by the CCK-8 method, and the Enzyme-linked immunosorbent assays (ELISA) and microplate method were used to measure the contents of matrix metalloproteinase, inflammatory and antioxidant factors in the photodamaged HaCaT cells. The antioxidant activity of RCP was detected by radical scavenging assays against D P P H radical (D P P H •) and ABTS radical (ABTS •
