Knockdown of NSUN2 Inhibits Melanoma Cells Proliferation by Regulating Cyclin Expression
10.13865/j.cnki.cjbmb.2022.06.1016
- Author:
Guo-Jin LI
1
;
Jin-Ping WANG
1
;
Qiang ZHANG
1
;
Yu-Wen JIAN
1
;
Shan-Shan WANG
1
;
Tao ZHANG
1
;
Ling WANG
1
;
Shan-Shan WANG
2
;
Tao ZHANG
3
;
Shan-Shan WANG
4
;
Tao ZHANG
4
;
Ling WANG
4
Author Information
1. School of Biological Science and Engineering, Shaanxi University of Technology, Department of Bioengineering
2. Shaanxi Key Laboratory of Bio-resources, Shaanxi University of Technology
3. QinLing-Bashan Mountains Bioresources Comprehensive Development C. I. C., Shaanxi University of Technology
4. Qinba State Key Laboratory of Biological Resources and Ecological Environment, Shaanxi University of Technology
- Publication Type:Journal Article
- Keywords:
gene knock down;
melanoma cells;
NOP2/Sun RNA methyltransferase 2 (Nsun2);
transcriptome sequencing
- From:
Chinese Journal of Biochemistry and Molecular Biology
2022;38(9):1242-1251
- CountryChina
- Language:Chinese
-
Abstract:
In order to investigate functions of NOP2/Sun RNA methyltransferase 2 (Nsun2) in melanoma cells, shRNA lentiviral vectors were constructed to target Nsun2 in mouse melanoma B16 cells. After treated B16 cells with recombinant virus, the mRNA and protein levels of NSUN2 in the interference group were significantly reduced, and the knock down efficiency reached 80%. EdU staining assay showed significant inhibition of DNA synthesis in Nsun2 knock-down B16 cells. RNA-seq was used to systematically analyze the gene expression of the Nsun2 knock-down and the control cells. A total of 1062 differentially expressed genes (DEGs) were screened, of which 678 were up-regulated and 384 were down-regulated. DEGs were mainly enriched in chromosome, centromere region, and protein binding GO terms. KEGG analysis showed that DEGs were significantly enriched in cell cycle, DNA replication, cell senescence and other pathways. RT-qPCR and RNA-seq data demonstrated that Cdk2, Ccnal, Cdc25b and other genes related to enhance cell division were significantly down-regulated, while Gadd45g and Gadd45a and other genes arresting cell growth were significantly up-regulated. Collectively, this study indicated that NSUN2 affects melanoma cell proliferation by regulating cell cycle and DNA replication, and provided fundamental data for exploring the molecular mechanism of melanoma genesis and development.
