EHD2 Affects the Proliferation of Esophageal Squamous Cell Carcinoma by Regulating the Cyclin Dl-CDK4-pRb Signaling Axis
10.13865/j.cnki.cjbmb.2022.08.1328
- Author:
Zhi-Da ZHANG
1
;
Dan-Xia DENG
1
;
Bing WEN
1
;
Liu PENG
1
;
Ke DONG
1
;
De-Yuan PAN
1
;
Lian-Di LIAO
1
;
Li-Yan XU
1
;
En-Min LI
1
;
Hai-Xiang ZHENG
2
;
Li-Yan XU
2
;
De-Yuan PAN
3
;
Li-Yan XU
3
Author Information
1. The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Department of Biochemistry and Molecular Biology, Shantou University Medical College
2. Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular lmmunopathology, Department of Pathology, Shantou University Medical College
3. Cancer Research Center, Shantou University Medical College
- Publication Type:Journal Article
- Keywords:
actin-binding protein (ABP);
Epsl5 homology domain-containing protein 2 (EHD2);
esophageal squamous cell carcinoma (ESCC);
proliferation;
T-cell factor 3 (TCF3)
- From:
Chinese Journal of Biochemistry and Molecular Biology
2022;38(9):1174-1192
- CountryChina
- Language:Chinese
-
Abstract:
Actin-binding proteins (ABPs) are important components of the F-actin cytoskeleton and affect the dynamics of F-actin by promoting the polymerization and depolymerization of actin. Numerous studies have shown that F-actin and actin-binding proteins are involved in all stages of carcinogenesis. Our analysis of esophageal carcinoma proteomic data showed that the actin-binding protein EHD2 (E p s l 5 homology domain-containing protein 2) is expressed at low levels in esophageal squamous cell carcinoma tissues and patients with lower EHD2 expression had poorer prognosis. Previous studies have revealed that EHD2 is involved in the regulation of glucose metabolism, autophagy and tumor cell migration. However, the role and mechanism of EHD2 in esophageal squamous cell carcinoma progression remain unclear. This study aimed to explore the effect of EHD2 on the proliferation of esophageal squamous cell carcinoma. Immunofluorescence and cell fractionation analysis showed that EHD2 was not only localized in the cell membrane and cytoplasm, but also in the nucleus. Colony formation, EdU labeling and flow cytometry were used to determine the effect of EHD2 on the proliferation of esophageal squamous cell carcinoma. The results showed that overexpression of EHD2 and EHD2-3×NLS (nuclear localization signal) inhibited proliferation, cell cycle G
