Establishment and Application of Visual LAMP Methods for QuickDetection of Vibrio Splendidus in Aquatic Products and Water
10.13865/j.cnki.cjbmb.2021.11.1366
- Author:
Zhuo TIAN
1
;
De-Jing SHANG
1
;
Ji-Juan CAO
1
;
Zhuo TIAN
2
;
Qiu-Yue ZHENG
2
;
Zhuo TIAN
3
;
Li-Dan MA
4
;
Wen-Jie ZHENG
5
Author Information
1. Cell Biology Major, College of Life Sciences, Liaoning Normal University
2. Key Laboratory of Biotechnologyand Resource Utilization of Ministry of Education, Dalian University for Nationalities
3. Dalian CustomsTechnology Center
4. Dandong Customs Comprehensive Technical Service Center
5. Biology Major, College of Life Sciences, Tianjin Normal University
- Publication Type:Journal Article
- Keywords:
aquaculture waters;
aquatic products;
loop-mediated isothermalamplification (LAMP);
Vibrio splendidus;
visual
- From:
Chinese Journal of Biochemistry and Molecular Biology
2022;38(1):108-119
- CountryChina
- Language:Chinese
-
Abstract:
A visualized, rapid, simple method was developed based on loop-mediated isothermal amplification (LAMP) to detect Vibrio splendidus in aquatic products and aquaculture waters. TakingVibrio splendidus as the research object, and the toxR gene of Vibrio splendidus as the target gene, the boiling method was determined as a fast method suitable for the genomic DNA extraction of Vibriosplendidus. The optimized and screened primers could specifically detect Vibrio splendidus, and the sensitivity of nucleic acid concentration detection could reach 10
