Ancient DNA Damage Analysis of Late Quaternary Mammalian Fossil Samples in Northeast China
10.13865/j.cnki.cjbmb.2022.03.1561
- Author:
Shi-Wen SONG
1
;
Gui-Lian SHENG
1
;
Miao-Xuan DENG
1
;
Xin-Dong HOU
1
;
Gui-Lian SHENG
2
;
Xu-Long LAI
2
;
Jun-Xia YUAN
3
;
Guo-Jiang SUN
3
;
Lin-Ying WANG
3
;
Bo XIAO
4
;
Jia-Ming HU
4
;
Xu-Long LAI
4
Author Information
1. School of Environmental Studies, China University of Geosciences (Wuhan)
2. State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences
3. Faculty of Materials Science and Chemistry, China University of Geosciences (Wuhan)
4. School of Earth Sciences, China University of Geosciences (Wuhan)
- Publication Type:Journal Article
- Keywords:
ancient DNA(aDNA);
next generation sequencing (NGS);
paleo-vertebrates;
quaternary
- From:
Chinese Journal of Biochemistry and Molecular Biology
2022;38(4):465-473
- CountryChina
- Language:Chinese
-
Abstract:
The advancement of the next generation sequencing (NGS) technology has promoted the development of ancient DNA research. Ancient DNA has made outstanding contributions in various fields such as human origin, animal evolution, etc. How to effectively extract and mine the genetic information from fossil and sub-fossil remains excavated from specific locations is a prerequisite for optimizing their important roles in many fields. In this study, we correlated the two main indicators of DNA damage (terminal base replacement rate, average fragment length) with the possible factors such as the burial time, geological epochs, tissue types, and sequencing library construction methods. The results show that the end base replacement rate of ancient DNA from Northeastern China is positively correlated with the water content of the environment and the ages of the samples. Among samples of different geological epochs, ancient DNA end base replacement rates have significant differences. On the contrary, different tissue types of the remains have no significant effects on the end base replacement rate of ancient DNA. The average fragment size of the molecules has no obvious correlation with the factors mentioned above. The results provide both solid data for investigating the characteristics of ancient DNA from specimens collected in Northeastern China, and valuable information for collecting appropriate samples from different geographical locations and the downstream storage before wet lab procedures after excavation.
