Hypoxic Preconditioning Improves Hypoxic Survival and Proliferation of Mesenchymal Stem Cells by Modulating Lysine Crotonylation
10.13865/j.cnki.cjbmb.2023.07.1135
- Author:
Jia-Hao YE
1
;
Chao-Yun LIU
1
;
Zhao PENG
1
;
Lei XIONG
1
;
Shao-Heng ZHANG
1
;
Shao-Heng ZHANG
2
;
Peng-Zhen WANG
3
;
Wen-Qian CAI
4
Author Information
1. Guizhou Medical University
2. Department of Cardiology, Guangzhou Red Cross Hospital, Medical College of Ji-Nan University
3. Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital, Medical College of Ji-Nan University
4. Heart Center, Guangzhou Institute of Pediatrics, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University
- Publication Type:Journal Article
- Keywords:
hypoxic preconditioning;
lysine crotonylation (Kcr);
peripheral blood mesenchymal stem cells (PBMSCs);
proliferation
- From:
Chinese Journal of Biochemistry and Molecular Biology
2023;39(8):1180-1190
- CountryChina
- Language:Chinese
-
Abstract:
Hypoxic preconditioning could improve the survival of mesenchymal stem cells (MSCs) in ischemic or hypoxic environments, but its exact mechanism remains to be further explored. This study aims to determine the role of lysine crotonylation (Kcr) in regulating the survival and proliferation of peripheral blood mesenchymal stem cells (PBMSCs) in the hypoxic culture. PBMSCs were isolated and cultured from rat peripheral blood mononuclear cells, and their surface markers were identified by flow cytometry. PBMSCs were first subjected to hypoxic/ normoxic preconditioning: hypoxic (1% O
