Inhibitory effect of recombinant human semaphorin 3A on angiogenesis of gastric cancer and the associated mechanisms
10.16098/j.issn.0529-1356.2020.02.012
- Author:
Pin FENG
1
;
Wen-Jing FAN
1
;
Lei LIU
2
;
Qian XU
3
;
Yu-Hong LI
4
;
Yan-Zhen ZUO
5
;
Bo ZHOU
6
;
Xiang-Yang ZHAO
7
Author Information
1. Department of Pathology, Chengde Medical University
2. Department of Immunology, Chengde Medical University
3. Basic Medical Institute, Chengde Medical University
4. Academic Affairs Office, Chengde Medical University
5. Department of Pharmacology, Chengde Medical University
6. Department of Neurology, 981 Hospital of Chinese PLA
7. Department of General Surgery, 981 Hospital of Chinese PLA
- Publication Type:Journal Article
- Keywords:
Co-culture system;
Gastric cancer;
Human;
Semaphorin3A;
Tube formation assay;
Umbilical vein endothelial cell;
Western blotting
- From:
Acta Anatomica Sinica
2020;51(2):220-227
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of semaphorin 3A (Sema3A) and its receptor neuropilin-1 (NRP-1) in gastric cancer and its correlation with microvessel density (MVD), and then to explore the effect of recombinant human Sema3A on angiogenesis of gastric cancer and the associated mechanisms. Methods Forty cases of gastric cancer tissues and its corresponding adjacent normal tissues were used to detecte the expression of Sema3A, NRP-1 and MVD in tissues by immunohistochemistry method . The expression level of Sema3A in serum of gastric cancer patient group and normal control group were measured by Enzyme-linked immuno-sorbent assay (ELISA). Western blotting was used to detect the expression of Sema3A and NRP-1 in five gastric cancer cell lines (MGC-803,HGC-27,MKN-28,SGC-7901,MKN-45) and human gastric mucosal epithelial cell (GES-1). Transwell chamber was used to construct non-contact in vitro co-culture system, in which the effects of different concentrations of recombinant human Sema3A on angiogenesis in gastric cancer were analyzed by tube formation assay preliminarily. The expression levels of vascular endothelial growth factor receptor 2 (VEGFR2) and NRP-1 in co-culture system were detected by Western blotting. Results The expression levels of Sema3A in gastric cancer tissues, cell lines and patient serum were significantly lower than that in the control group(P<0. 05), while the expression of NRP-1 in gastric cancer tissues and MKN-28 cells was significantly increased, and both of them were associated with TNM staging of gastric cancer (P < 0. 05) . In vitro co-culture system, The tube forming abilities of human umbilical vein endothelial cell (HUVEC) were decreased in recombinant human Sema3A treated group, and this phenomenon was concentration dependent. The expression of VEGFR2 protein was down-regulated by recombinant human Sema3A. Conclusion The expression of Sema3A was decreased in gastric cancer tissues, cell lines and patient serum, and negatively correlated with microvessel density. The recombinant human Sema3A could inhibit the angiogenesis of gastric cancer in vitro, which may be related to down-regulation of VEGFR2 protein expression.
