Synthesis of five types of nucleobase-alanines and their mechanisms of inhibiting proliferation of rat hepatoma cells RH35
10.16098/j.issn.0529-1356.2020.02.008
- Author:
Cun-Shuan XU
1
;
Ya-Ping SONG
1
;
Kai XU
1
;
Hui YANG
1
;
Yi DING
1
;
Jing-Jing BI
2
;
Qian-Yi ZHAO
2
;
Ting XU
2
Author Information
1. State Key Laboratory Cultivation Base for Cell Differentiation Regulation, School of Life Science, He’nan Normal University
2. School of Chemistry and Chemical Engineering, He’nan Normal University
- Publication Type:Journal Article
- Keywords:
Cell proliferation kinetics;
Cytotoxicity;
Flow cytometry;
Nucleobase-alanine;
Rat
- From:
Acta Anatomica Sinica
2020;51(2):195-205
- CountryChina
- Language:Chinese
-
Abstract:
Objective Five types of nucleobase-alanines were synthesized to study their cytotoxicity and mechanism. Methods Five types of nucleobase-alanines were synthesized by the method of nucleophilic ring-opening of serine-lactone. MTT assay was used to detect their effects on the viability of rat normal hepatocytes BRL-3A and rat hepatoma cells RH35. Flow cytometry was used to detect their effects of cell cycle and apoptosis on BLR-3A and RH35. Real-time PCR was performed to detect their effects of proliferation/ apoptosis-related gene expression on these two cells. Results Five types of nucleobase-alanines were synthesized, including 1-uracil-L-alanine, 1-thymine-L-alanine, 1-cytosine-L-alanine, 9-adenine-L-alanine and 9-guanine-L-alanine. Among them, 1-cytosine-L-alanine and 9-guanine-L-alanine had significant lower half inhibition concentration(IC
