Distribution characteristics of polymorphism of miRNA-107 gene in Guangxi population and their association with serum lipid levels
10.16098/j.issn.0529-1356.2020.02.022
- Author:
Yan-Yun HUANG
1
;
Hua-Tuo HUANG
1
;
Yu-Lan LU
1
;
Ming LEI
1
;
Tan TAN
1
;
Ye-Sheng WEI
1
;
Yan-Yun HUANG
2
Author Information
1. Department of Clinical Laboratory, Affiliated Hospital of Youjiang Medical University for Nationalities
2. Department of Clinical Laboratory, People’s Hospital of Baise
- Publication Type:Journal Article
- Keywords:
DNA sequencing;
Ethnic;
Guangxi population;
Lipoprotein;
MiR-107 gene;
Multiplex SNaPshot;
rs2296616 C/T;
Single nucleotide polymorphism
- From:
Acta Anatomica Sinica
2020;51(2):284-288
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the distribution characteristics of single nucleotide polymorphisms (SNP) of miR-107 gene rs2296616 C/T in Guangxi healthy population and comparison with that in different ethnic populations, and further to explore the correlation between rs2296616 C/T SNP and blood lipid level. Methods The polymorphisms of miR-107 gene rs2296616 C/T among 372 Chinese healthy individuals of Guangxi were detected by multiplex SNaPshot and DNA sequencing method, and the blood lipid-related indexes were detected by 7600 biochemical analyzer. The distribution of rs2296616 C/T polymorphism among different ethnic groups and the differences of blood lipid levels among different genotypes were compared by statistical method . Results MiR-107 gene rs2296616 C/T SNP contained TT(91. 1%), CT (8. 9%)genotypes and T(95. 6%), C(4. 4%)alleles in Guangxi healthy population. The frequencies of genotype and allele distribution of rs2296616 C/T were not significantly different among genders in Guangxi population(P>0. 05). However, there were significant differences in the genotype and allele frequency of miR-107 gene rs2296616 C/T in Guangxi healthy population compared with those of Europeans, Japanese, Africans, Mexicans and Indians published in HapMap(P<0. 05), no significant difference compared with HapMap-HGB (P > 0. 05). When compared the blood lipid level among two genotypes in rs2296616 C/T, we found that the level of high density lipoprotein cholesterol(HDL-C) with TT genotype was significantly different from that of CT group (P < 0. 05) . Conclusion There are different degrees of variation in the polymorphisms of rs2296616 C / T of miR-107 gene between Guangxi people and other ethnic populations. The polymorphism of rs2296616 C / T locus is related to the level of HDL-C.
