Sudan black B reducing the intestinal autofluorescence of rats and its application in immunofluorescence
10.16098/j.issn.0529-1356.2020.03.024
- Author:
Yong LI
1
;
Li ZHOU
1
;
Yang ZHANG
1
;
Feng-Ge SHEN
1
;
Zhi-Yong WANG
1
;
Zhen LIU
2
;
Nian YAO
2
Author Information
1. Department of Human Anatomy, Xinxiang Medical University
2. School of Basic Medical Sciences, Xinxiang Medical University
- Publication Type:Journal Article
- Keywords:
Autofluorescence;
Immunofluorescence;
Intestine;
Rat;
Sudan black B
- From:
Acta Anatomica Sinica
2020;51(3):450-455
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a feasible method to reduce the rat intestinal autofluorescence (AF). Methods Five rats were sacrificed. After cryostat section, intestinal AF was investigated by immunofluorescence (IF) before and after Sudan black B (SBB) treatment. Results The AF within the different rat intestinal segments was easily detected in both FITC- and TRITC- channel spectrum, which was not affected by serum blocking. After 0. 3% SBB treatment for 10 minutes, a significant reduction of AF was observed in the rat intestinal tissue. Moreover, the SBB treatment did not affect the expression of CD45 in the rat intestine detected by IF staining, but reduced the AF remarkedly. Conclusion The AF in rat intestinal frozen section can be effectively reduced with 0. 3% SBB treatment for 10 minutes.
