Effects of dihydrotestosterone on the expression of anti-Müllerian hormone in primary follicular granulosa cells in rats
10.16098/j.issn.0529-1356.2020.03.009
- Author:
Mei-Ling WANG
1
;
Yu-Ting DONG
1
;
Chang-Chun HEI
1
;
Yu-Fang CAI
1
;
Bin KONG
1
;
Cheng-Jun ZHAO
1
;
Qing CHANG
1
;
Xiao-Feng YE
2
Author Information
1. Department of Human Anatomy, Histology and Embryology, School of Basic Medicine, Ningxia Medical University, Fertility Conservation Key Laboratory of Ministry of Education
2. Second Department of Oncology, Cancer Hospital of General Hospital of Ningxia Medical University
- Publication Type:Journal Article
- Keywords:
Anti-Müllerian hormone;
Dihydrotestosterone;
Follicular granulosa cell;
Immunofluorescence;
Rat;
Western blotting
- From:
Acta Anatomica Sinica
2020;51(3):367-372
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore how dihydrotestosterone (DHT) affects the expression of anti-Müllerian hormone (AMH) in primary rat follicular granulosa cells, and then provides a new experimental basis for the role of DHT and AMH in follicular development. Methods Granulosa cells was extracted from the ovaries of 95 21 days SD female rats for primary culture. Firstly, cell morphology were detected by HE staining and follicke stimulating hormone receptor (FSHR) immunofluorescence detected cell purity after 48 hours of cell culture. Then, cells were randomly divided into control group (without drug intervention), 10
