Effects of microRNAs on invasion, migration and apoptosis of lung cancer cell lines
10.16098/j.issn.0529-1356.2020.03.013
- Author:
Jing-Jun HAN
1
;
Lei ZHANG
1
;
Ying-Jie XU
1
;
Hai-Zhou LI
1
;
Yan-Wei ZHANG
2
;
Shu-Guang LIU
3
Author Information
1. Department of Cardiothoracic Surgery, the Eighth Affiliated Hospital of Sun Yat￣sen University
2. Institute of Immunization, Shenzhen Center for Disease Control and Prevention
3. Department of Pathology, the Eighth Affiliated Hospital of Sun Yat￣sen University
- Publication Type:Journal Article
- Keywords:
Invasion;
Lung cancer cell line;
MicroRNA;
Migration;
Real-time PCR
- From:
Acta Anatomica Sinica
2020;51(3):392-397
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of microRNAs(miRNAs) on invasion, migration and apoptosis of lung cancer cell lines. Methods Target cells were infected with hsa-mir-933, hsa-mir-4700-3p, hsa-mir-3144-3p, hsamir-3972, and hsa-mir-548a-5p. Cell apoptosis was measured using flow cytomertry, Transwell invasion assay, cell migration were analyzed by lineation,miRNA was quantified using Real-time PCR. Results Compared with the vector and control, in hsa-mir-933, hsa-mir-4700-3p, hsa-mir-3144-3p, hsa-mir-397 and hsa-mir-548a-5p group the apoptosis significantly increased,cell invasion ability significantly decreased,cell migration ability significantly decreased, miRNAs expression significantly increased. Conclusion Five microRNAs can promote the apoptosis of lung cancer cell lines, change the high invasiveness of lung cancer cell lines, inhibit the migration of lung cancer cell lines, and increase the expression of corresponding miRNAs.
