Expression of miR-145-5p in colorectal cancer and its relationship with multidrug resistance
10.16098/j.issn.0529-1356.2020.04.013
- Author:
Ya-Nan YUAN
1
;
Meng-Nan DU
1
;
Xiao-Li YAN
1
;
Yan-Jie HUANG
1
;
Ji-Ning ZHENG
1
Author Information
1. Department of Pathology and Pathophysiology, Chengde Medical University
- Publication Type:Journal Article
- Keywords:
Colorectal carcinoma;
Human;
Immunohistochemistry;
MiR-145-5p;
Multidrug resistance;
P-glycoprotein;
Real-time PCR;
Western blotting
- From:
Acta Anatomica Sinica
2020;51(4):553-556
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and relationship between miR-145-5p and multidrug resistance gene (MDR1) protein P-glycoprotein(P-gp) in colorectal cancer. Methods SP, Real-time PCR and Western blotting were used to detect the expression of P-gp colorectal carcinoma (CRC) in 50 patients and in 30 paracancerous normal tissue patients, the effect of miR-145-5p expression on MDR1 mRNA and P-gp and the correlation between them and clinicopathological features. Results The expression level of miR-145-5p in CRC was significantly lower than that in paracancerous normal tissue, and the expression level of MDR1 mRNA and P-gp was significantly higher than that in paracancerous normal tissue (r=-0. 403, P<0. 01).MiR-145-5p could inhibit the expression of MDR1 mRNA and P-gp in HCT-15 cells(P<0. 05). Conclusion MiR-145-5p plays a regulatory role in the expression of multidrug resistance genes and proteins in colorectal cancer, and is involved in the occurrence, development and multidrug resistance of colorectal cancer.
