Quality control analysis of brain tissue samples from Shanghai Brain Bank
10.16098/j.issn.0529-1356.2021.02.002
- Author:
Feng-Jiao LI
1
;
Wei-Wei XIAN
1
;
Jing-Li CAO
1
;
Wen-Sheng LI
1
;
Lin-Ya YOU
1
;
Shuo-Lei BU
2
;
Jin-Song WU
3
;
Lin-Ya YOU
4
Author Information
1. Department of Human Anatomy and Histoembryology, School of Basic Medical Sciences, Fudan University
2. Institutes of Biomedical Sciences, Fudan University
3. Department of Neurosurgery, Huashan Hospital, Fudan University
4. Shanghai Key Laboratory of Medical Imaging Computing and Computer Assisted Intervention
- Publication Type:Journal Article
- Keywords:
Brain tissue;
HE staining;
Human;
pH value of cerebrospinal fluid;
Protein;
Ribonucleic acid
- From:
Acta Anatomica Sinica
2021;52(2):168-174
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the feasibility of using brain tissues from Shanghai Brain Bank for the applications on biological research through the analysis of pH value of cerebrospinal fluids, RNA integrity number (RIN), transcriptome, proteome and morphology of brain tissues. Methods The pH value of fresh cerebrospinal fluid was detected by pH test paper; the RNA integrity of cryopreserved brain tissues was examined by Agilent RNA 6000 Nano chip and Agilent 2100 bioanalyzer; the transcriptome sequencing of superior temporal gyrus or caudate was performed using BGIseq-500 sequencer; the proteome of cryopreserved brain tissues was analyzed by Q Exactive HF mass spectrometer; at last, the morphology of the superior temporal gyrus was observed by HE staining. Results The pH value of the cerebrospinal fluid on average was about 6.5. The RIN values of more than 65% of brain tissues were more than 6, indicating good RNA quality. The clean reads ratio after transcriptome sequencing filtering was basically above 80%, indicating that the quality of sequencing library was high. The mass spectrometry analysis of frozen brain samples yielded more than 4000 protein groups and 30 000 peptides, indicating high quality of proteomic data. The morphology of brain tissues was relatively normal, with clearly visible neurons. Conclusion The quality of RNA and protein of brain tissues from Shanghai Brain Bank meets the basic needs for molecular and biological research, and the fixed brain samples can be used for morphological observations.
