Dynamic expression of putative receptor protein related to the angiotensin type 1 receptor in postnatal mouse cochlear hair cells and spiral ganglion neurons
10.16098/j.issn.0529-1356.2021.02.003
- Author:
Hai-Yan YIN
1
;
Ze-Yu WANG
1
;
Chang-Jiang HAO
1
;
Hui ZHANG
1
;
Feng-Qin WANG
1
;
Xiu-Guo LI
2
;
Jing CHEN
3
Author Information
1. Department of Histology and Embryology, Jining Medical University
2. Otolaryngology-Head and Neck Surgery, Jining First People’s Hospital
3. Institute of Neurobiology, Jining Medical University
- Publication Type:Journal Article
- Keywords:
Hair cell;
Immunofluorescence;
Mouse;
Putative receptor protein related to angiotensin type 1 receptor;
Spiral ganglion neuron
- From:
Acta Anatomica Sinica
2021;52(2):175-181
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the temporal and spatial expressions of G protein-coupled receptor, putative receptor protein related to angiotensin type 1 receptor (APJ), in mammal cochlea postnatal development. Methods The cochlear tissues of each group 11 C57BL/ 6 mice at postnatal day 7 (P7), P14, P28 and postnatal month 2(P2M) were taken out under a stereo microscope. Real-time PCR, Western blotting and immunofluorescent staining were used to detect the expressions of APJ in hair cells and spiral ganglion neurons. Results The expression pattern of APJ in cochleae showed an upward trend during the period from P7 to P2M. The temporal expressions of APJ in hair cells and spiral ganglion neurons increased obviously at P14 and P2M. The spatial expression patterns of APJ in hair cells and spiral ganglion neurons followed a declined gradient from base turns to apex turns at P14. Conclusion APJ expression exhibits a specific spatial and temporal pattern during mouse cochlea postnatal development, and may play a role in cochleae maturation and hearing formation.
