MicroRNA-98-5p targeting ribonucleotide reductase small subunit M2 regulating cisplatin resistance in cervical cancer cells
10.16098/j.issn.0529-1356.2021.03.016
- Author:
Xia ZHANG
1
;
Zhi-Ming JIANG
2
;
Xiao-Yan CHENG
2
;
Yan XIANG
2
;
Xiao ZHAO
2
;
Yu-Ping HUANG
2
;
Hao YU
2
Author Information
1. Department of Gynecology, Tianxiang Oriental Hospital
2. Department of Gynecology, Lishui Maternal and Child Health Hospital
- Publication Type:Journal Article
- Keywords:
Cervical cancer;
Cisplatin;
Human;
Micro RNA-98-5p;
Ribonucleotide reductase small subunit M2;
Western blotting
- From:
Acta Anatomica Sinica
2021;52(3):432-438
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulation and mechanism of microRNA (miR)-98-5p on cisplatin sensitivity in cisplatin-resistant cervical cancer cells. Methods The cisplatin(DDP) +miR-NC group (transfected miR- NC), DDP + miR-98-5p group (transfected miR-98-5p mimics), DDP + si-NC group (transfected si-NC), DDP + si- ribonucleotide reductase subunit M2 (RRM2) group (transfected si-RRM2), DDP + miR-98-5p + pcDNA group (co- transfected miR-98-5p mimics and pcDNA), DDP + miR-98-5p + pcDNA-RRM2 group (co-transfected miR-98-5p mimics and pcDNA-RRM2) were transfected into HeLa/DDP cells by liposome method. Real-tim PCR, Western blotting, CCK-8, Transwell chamber and dual luciferase reports gene detection assay were used to detect the expression of miR-98-5p, RRM2, cyclin Dl, P21, matrix metalloproteinase (MMP)-2 and MMP-9 in cells, inhibition rate, half inhibitory concentration(IC
