Targeted quantitative analysis of energy metabolites in the priming phase during rat liver regeneration
10.16098/j.issn.0529-1356.2021.03.008
- Author:
Hui YANG
1
;
Cun-Shuan XU
1
;
Hui YANG
2
;
Cun-Shuan XU
2
Author Information
1. College of Life Science, Henan Normal University
2. State Key Laboratory Cultivation Base for Cell Differentiation Regulation, Henan Normal University
- Publication Type:Journal Article
- Keywords:
Energy metabolism;
Hierarchical clustering;
Ingenuity Pathway Analysis;
Liver regeneration;
Rat;
Targeted metabolomics
- From:
Acta Anatomica Sinica
2021;52(3):377-383
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulation of liver regeneration (LR) by changes in energy metabolites in the initiation phase during rat liver regeneration. Methods Rats were randomly divided into 3 groups with 5 rats in each group, including two partial hepatectomy (PH) groups and one normal control group. Selective reaction monitoring/multiple reaction monitoring (SRM/MRM) was employed in the targeted metabolomics identification of 29 energy metabolites. Ingenuity Pathway Analysis (IPA) was applied for integration analysis, including canonical pathway and molecular interaction network. Results The levels of 3-phospho-D-glycerate, AMP, cyclic AMP, D-fructose 1, 6-bisphosphate, dihydroxyacetome phosphate (DHAP), guanosine monophosphate (GMP), guanosine triphosphate (GTP), nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinueleotide phosphate ( NADP ) significantly increased. The levels of alpha-ketoglutarate, beta-D-fructose 6-phosphate, cis-aconitate, D-glucose 6-phosphate, lactate, NADPH, oxaloacetate and pyruvate dramatically reduced. Through hierarchical clustering analysis of energy metabolisms, these energy metabolisms can be grouped into four clusters. IPA showed that the biomolecular changes in the priming phase of liver regeneration are mainly related to carbohydrate metabolism, cellular growth and proliferation, and organismal development. During the priming phase of liver regeneration, adenosine 5'-monphosphate-activated protein kinase (AMPK), hypoxia- inducible factor la (HIF-la), peroxisome proliferator-activated receptor (PPAR), protein kinase A (PKA) and phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) signaling pathways are involved in energy metabolism, and glycolysis may be the main mode of energy supply. Conclusion The result suggests that the changes of energy matabolites during the initial stage of LR play a regulatory role in live regeneration.
