Screening and identification the hub genes of cardiac hypertrophy in mice
10.16098/j.issn.0529-1356.2021.03.010
- Author:
Zhuo SHI
1
;
Fu REN
1
;
Zhuo SHI
2
;
Fu REN
2
;
Pu-Shuai WEN
2
;
Zhuo SHI
3
;
Jia-Hui LI
4
;
Jing GAO
5
;
Yan-Li DONG
6
;
Pu-Shuai WEN
7
Author Information
1. Department of Anatomy, School of Basic Medical Science, Jinzhou Medical University
2. Liaoning Key Research Laboratory of Human Phenotype Group, Jinzhou Medical University
3. Department of Nursing, Jinzhou Health School
4. The Third Clinical Medical College, Jinzhou Medical University
5. Department of Ultrasound, The First Affiliated Hospital of Jinzhou Medical University
6. Nursing Group of Experimental Center, Chaoyang Health School
7. Department of Pathophysiology, School of Basic Medical Science, Jinzhou Medical University
- Publication Type:Journal Article
- Keywords:
Bioinformatics;
Cardiac hypertrophy;
Differentially expressed gene;
Kunming mouse;
Real-time PCR
- From:
Acta Anatomica Sinica
2021;52(3):391-397
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen and identify the hub genes closely related to cardiac hypertrophy by using bioinformaticsmethod and biological experiments. Methods The chip data related to cardiac hypertrophy in mice were downloaded from the Gene Expression Omnibus (GEO) database, and the GE02R online tool was adopted to screen for differentially expressed genes; DAVID 6.7, String 11.0 and Cytoscape 3.7. 0 softwares were used to analyze differentially expressed genes; Kunming mice were randomly divided into a normal saline group (n = 6) and an angiotensin II (Ang II) group (n = 6) to establish a cardiac hypertrophy model, the expression of hub gene in Kunming mouse model of cardiac hypertrophy induced by Ang II was detected by Real-time PCR method. Results A total of 202 common differentially expressed genes and 12 hub genes were selected; the Real-time PCR result demonstrated that decorin(Dcn), HADHA and heat shock protein (HSP) 90αA 1 were significantly down-regulated in the Angli group. Conclusion The selected hub genes can influence the development of cardiac hypertrophy in Kunming mice through extracellular matrix and transforming growth factor β(TGF-β).
