Construction of corneal neovascularization rabbit model by suture
10.16098/j.issn.0529-1356.2021.04.024
- Author:
Li-Qun DU
1
;
Jia-Zhang SUN
1
;
Feng-Hua MI
1
;
Xin-Yi WU
1
Author Information
1. Department of Ophthalmology, Qilu Hospital, Shandong University
- Publication Type:Journal Article
- Keywords:
Cornea;
Enzyme-linked immunoassay;
HE staining;
Immunofluoresence;
Neovascularization;
Rabbit;
Sew up;
Suture
- From:
Acta Anatomica Sinica
2021;52(4):657-661
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the effect of different sutures and suture method on corneal neovascularization ( CNV) in rabbit models. Methods NV was induced by placing sutures at the corneal periphery of rabbits (n = 45). To observe the NV status, 45 rabbits were randomly divided into 5 equal groups. Group A applied 8-0 absorbable suture (A1 single loop parallel suture, A2 single loop vertical suture). In group B, 10-0 nylon suture was used (B1 double loop parallel suture, B2 double loop vertical suture, B3 three loop radial suture). The development of CNV was observed with slit lamp microscope and photographed. Therefore the effective model for neovascularization induction was selected. Histological examination, immunofluorescent staining and ELISA analysis for the vascular endothelial growth factor( VEGF) were performed before suture, 7 and 14 days after suture. Results Sutures fell off and CNV gradually atrophied in group Al and A2; At the 14th day after suture, Sparse or short cluster CNV grew into the corneal margin in group B1 and B2, while CNV was vigorous and grew in bundles in group B3. The expression of VEGF in aqueous humor increased in B3 group after suturing, and increased in 14 days as compared with 7 days after suture. Corneal edema, neovascularization and little immunofluorescence staining for VEGF were detected in group B3 after 7 days suture. More neovascularization and immunofluorescence staining for VEGF were detected in group B3 after 14 days suture. Conclusion Corneal NV can be induced successfully in rabbit model by suturing. The method of 10-0 thread with three sets of circular seams (B3) is stable and effective.
