Effect and mechanism of fibroblast growth factor on proliferation and transdifferentiation of cardiac fibroblasts in rats
10.16098/j.issn.0529-1356.2021.05.007
- Author:
Meng-Xin LIU
1
;
Chang-Ye SUN
1
;
Duo LI
1
;
Zhi-Kun GUO
1
;
Ming-Fen REN
2
Author Information
1. He' nan Key Laboratory of Medical Tissue Regeneration of Xinxiang Medical University
2. Department of Cardiology, The Second Affiliated Hospital of Xinxiang Medical University
- Publication Type:Journal Article
- Keywords:
a-Smooth muscle actin;
Cardiac fibroblast;
Fibroblast growth factor;
Myofibroblast;
Western blotting
- From:
Acta Anatomica Sinica
2021;52(5):712-719
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of fibroblast growth factor (FGF) on the proliferation and transdifferentiation of cardiac fibroblasts ( CFs ) into myofibroblasts ( MFs ). Methods Rat CFs were isolated and cultured, and then induced by FGF. CCK-8 was used to detect the cell activity and proliferation. Immunofluorescence and Western blotting were used to detect the expression of a smooth muscle actin ( α-SMA ) and collagen I ( Col I ). Results The expression and activation of α-SMA and Col I increased with the increase of CFs culture generation. The number of CFs induced by FGF did not increased significantly; the expression of α-SMA in CFs induced by FGF1 and FGF2 decreased, and the number of activated MFs decreased. Conclusion FGF family has no effect on the proliferation of CFs, but FGF1 and FGF2 can inhibit the activation of CFs and reduce the differentiation into MFs.
