Effects of velvet antler polypeptide on Rho/ROCK pathway in APP/ PSl double transgenic mice
10.16098/j.issn.0529-1356.2022.02.008
- Author:
Zhong-Jin LIU
1
;
Chun-Mei ZHANG
1
;
Yu-Di ZHANG
1
;
Tong-Hui YI
2
;
Wei-Ya LANG
2
;
Hai-Yan ZHANG
2
Author Information
1. Department ofNeurology, The First Hospital Affiliated of Qiqihar Medical College
2. Department of Histology and Embryology, Basic Medical College of Qiqihar Medical University
- Publication Type:Journal Article
- Keywords:
Alzheimer' s disease;
Mouse;
Ras homology gen famity member/Rho associated coiled-coil forming protein pathway;
Synapse;
Velvet antler polypeptides;
Western blotting
- From:
Acta Anatomica Sinica
2022;53(2):190-195
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of velvet antler polypeptides (VAP) on Rho/ROCK pathway in APP/ PSl double transgenic mice. Methods APP/PSl double transgenic mice were randomly divided into model group and velvet antler polypeptide group, 20 mice in each group, and control group consisting of 20 mice of the same litter and the same gender negative. The mice in VAP group were given velvet antler polypeptide 100 mg/kg by intragastric administration once a day for 28 days. After treatment, the water maze experiment was detected and recorded the escape latency and the number of crossing platforms of the mice; the ultrastructures of the synapse were observed by transmission electron microscopy; the expression of Rhs homolog gene family member A(RhoA) and Rho associated coiled-coil forming protein kinase II(ROCKII) in the hippocampal CAI area were observed by immunofluorescence. The expression levels of RhoA and ROCKII protein in the hippocampus were detected by Western blotting. The contents of hippocampus amyloid (3-protein(A(3),
