Molecular mechanism of microRNA-29a-3p targeting Serpinhl regulating proliferation and invasion of human gastric cancer cell line BGC823
10.16098/j.issn.0529-1356.2022.05.010
- Author:
Xiao-Di YAN
1
;
Qiang XUE
1
;
Fei-Ran WANG
1
;
Hong-Mei GU
1
;
You-Lang ZHOU
1
;
Xian-Cheng LIU
1
;
Chong TANG
2
;
Rui-Qi LIU
3
Author Information
1. Department of Cancer Center, Affiliated Hospital of Nantong University
2. Department of Gastrointestinal Surgery, Nantong First Peoples Hospital
3. Nantong Univeristy Medical School, Grade 2021
- Publication Type:Journal Article
- Keywords:
Gastric cancer;
Invasion;
MicroRNA-29a-3p;
Proliferation;
Serpinhl;
Western blotting
- From:
Acta Anatomica Sinica
2022;53(5):607-612
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of microRNA( miR)-29a-3p on the proliferation and invasion of gastric cancer cells and analyze its related molecular mechanism. Methods The expression level of miR-29a-3p in gastric cancer cells was detected, and the role of miR-29a-3p in the proliferation, migration, and invasion of gastric cancer cells was evaluated. Western blotting and luciferase analysis showed that miR-29a-3p was directly bound to Serpinhl 3 ' -untranslated region(3' UTR). In addition, the effects of the miR-29a-3p/Serpinhl axis on the proliferation, migration, and invasion of gastric cancer cells were detected by MTT assay, colony formation assay, and Transwell assay in vitro. Results After transfection, the expression of miR-29a-3p in the miR-29a-3p mimic group was significantly higher than that in the miR-29a-3p negative control and blank group. After transfection, the proliferation of BGC823 cells decreased significantly. Luciferase analysis showed that miR-29a-3p inhibited the expression of Serpinhl by targeting the 3 ' UTR of Serpinhl. In addition, overexpression of miR-29a-3p significantly inhibited the proliferation, invasion, and migration of gastric cancer cells by targeting Serpinhl. Conclusion MiR-29a-3p can target Serpinhl and regulate the proliferation and invasion of gastric cancer cells.
