Shear stress regulateing the phosphorylation of endothelial nitric oxide synthase Ser633 and Ser1177 in human umbilical vein endothelial cells through Pim1 / Akt pathway
10.16098/j.issn.0529-1356.2023.05.007
- Author:
Da-Peng DU
1
;
Han-Qin WANG
1
;
Da-Peng DU
2
;
Yu SUN
2
;
Min ZHANG
2
;
Han-Qin WANG
2
Author Information
1. Department of Anatomy, Basic Medical College, Hubei University of Medicine
2. Center for Translational Medicine, Suizhou Hospital, Hubei University of Medicine
- Publication Type:Journal Article
- Keywords:
Endothelial cell;
Endothelial nitric oxide synthase;
Human;
Pim1;
Shear stress;
Western blotting
- From:
Acta Anatomica Sinica
2023;54(5):546-552
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective To explore the effect of different modes of blood flow shear stress on the Pim1 expression in human umbilical vein endothelial cells (HUVECs) and the regulation of phosphorylation at Ser1177 and Ser633 of endothelial nitric oxide synthase (eNOS). Methods HUVECs were isolated from fresh human umbilical cord. The parallel plate flow chamber system was used to load 15 dyn/ cm
