Effect of serine protease inhibitor Kazal-type 1 on the proliferation of hepatocellular carcinoma cell and its mechanism
10.16098/j.issn.0529-1356.2023.06.011
- Author:
Wei-Ming ZHAO
1
;
Xiao LI
1
;
Guo-Ying YU
1
;
Gai-Ping WANG
1
;
Cui-Fang CHANG
1
Author Information
1. Collage of Life Sciences, He’nan Normal University, State Key Laboratory for Cell Differentiation and Regulation
- Publication Type:Journal Article
- Keywords:
Cell proliferation;
Flow cytometry;
Human;
Liver cancer;
Rat;
Serine protease inhibitor Kazal-type 1
- From:
Acta Anatomica Sinica
2023;54(6):695-702
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective To explore the effect of serine protease inhibitor Kazal-type 1(SPINK1) on the proliferation of hepatocellular carcinoma cells RH-35 and its underling molecular mechanism. Methods Spink1 gene expression in liver cancer and rat liver cancer models were analyzed by Gene Expression Omnibus (GEO) data, RH-35 cells were treated with rrSPINK1 protein, the effect of rrSPINK1 on the proliferation and apoptosis of RH-35 cells was explored by MTT, 2’-deoxy-5-ethynyluridine(EdU) and flow cytometry, the molecular mechanism of SPINK1 regulating liver cancer were detected by Real-time PCR and Western blotting. Results The results showed that Spink1 gene was over-expressed significantly in liver cancer and rat liver cancer models, rrSPINK1-treated RH-35 cells showed increased viability, EdUpositive cell rate, and the proportion of cells in S phase and G
