Chronopharmacokinetics of melatonin in non-dipper spontaneous hypertension rats
10.12092/j.issn.1009-2501.2020.12.003
- Author:
Haiju CUI
1
;
Zhangqing MA
2
;
Wusan WANG
2
;
Kui YANG
3
;
Huifang XU
3
;
Jiajie LUAN
3
Author Information
1. Department of Pharmacy, Xuancheng Vocational and Technical College
2. School of Pharmacy, Wannan Medical College
3. Department of Pharmacy, The First Affiliated Hospital of Wannan Medical College
- Publication Type:Journal Article
- Keywords:
Circadian rhythm;
Melatonin;
Non-dipper hypertension;
Pharmacokinetics
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2020;25(12):1337-1343
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the chronological pharmacokinetic differences of melatonin (MEL) in non-dipper spontaneously hypertensive rats (SHR). METHODS: The HPLC detection method of MEL was established, and the specificity, precision, recovery rate and stability of the method were examined. Twelve male SD rats were divided into two groups, and a single dose of MEL (20 mg/kg) was given intragastrically at either 08:00 or 20:00, respectively. Plasma samples were collected at 0, 5, 10, 15, 20, 30, 40, 60, 90, 120, 240, 360 min after drug administration, and the plasma MEL concentration was determined by fluorescence HPLC. RESULTS: The specificity, precision, recovery rate and stability of the MEL detection method established in this study were in line with the requirements of the biological analysis method guidelines, proving that the method was mature and reliable. After MEL was administered at 08:00, the T
