Preparation and the effect of antitumor of DNA plasmid lipidosome vaccine based VEGFR2 extracellular region by immunization activated in vitro
10.12092/j.issn.1009-2501.2021.01.004
- Author:
Minfang CHEN
1
;
Liyun XIE
2
;
Fenfen XU
2
;
Aixiao XIA
2
;
Zhong LIN
2
;
Lihua CHEN
3
Author Information
1. Department of Thoracic Surgery, Wenling Traditional Chinese Medicine Hospital
2. Clinical Pharmacy Laboratory, Taizhou Hospital of Zhejiang Province
3. Public Experimental Platform, Taizhou Hospital of Zhejiang Province
- Publication Type:Journal Article
- Keywords:
Lipidosome;
PCMV plasmid;
Vaccine;
VEGFR2
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2021;26(1):24-29
- CountryChina
- Language:Chinese
-
Abstract:
AIM: The DNA plasmid lipidosome (LP) vaccine based VEGFR2 extracellular region (exVEGFR2) was prepared in order to provide a new approach for cancer active immunotherapy. METHODS: High fidelity PCR was used to amplify the target sequence of exVEGFR2 with two restriction site of Kpn and Xba. The plasmid of pCMV/exVEGFR2 was constructed by connected exVEGFR2 with pCMV empty plasmid. The activity of immune activation was detected by ELISA. CTLs mediated cytotoxic activity was analyzed by
