Tangshen formula promotes cellular cholesterol efflux in HG-induced mTECs by suppression of TGF-β1/Smad3 pathway
10.12092/j.issn.1009-2501.2021.01.001
- Author:
Peng LIU
1
;
Zhengri SHEN
1
;
Chen WANG
1
;
Yun WANG
1
;
Xinping QIU
1
;
Hailing ZHAO
2
;
Ping LI
2
Author Information
1. hunyi Hospital, Beijing Traditional Chinese Medicine Hospital
2. Beijing Key Lab for Immol/Lune-Mediated Inflammatory Diseases, Institute of Clinical Medical Science, China-Japan Friendship Hospital
- Publication Type:Journal Article
- Keywords:
Cellular cholesterol efflux;
Diabetic nephropathy;
SIS3 (Smad3 inhibitor);
Tangshen formula;
TGF-β1/Smad3 pathway
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2021;26(1):1-9
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the effect of Tangshen formula (TSF) treatment on TGF-β1/Smad3 pathway and cellular cholesterol efflux and explore its potential mechanism in HG-induced mouse tubular epithelial cells (mTECs). METHODS: After 25 mmol/L high glucose induced mTECs, TSF and Smad3 inhibitor SIS3 were given to intervene respectively. The lipid content in the cells was detected by ELISA kit; TGF-β1/Smad3 pathway and PGC-1α, LXR, ABCA1, ABCG1 were detected by Western blot and real-time PCR. RESULTS: TSF diminished the levels of TC, TG, LDL-C and increased the levels of HDL-C in HG-induced mTECs. Western blot and real-time PCR analysis showed that expression levels of TGF-β1, Smad3, Collagen and Fibronectin were significantly downregulated in the HG-induced mTECs with TSF and SIS3 treatment. And PGC-1α, LXR, ABCA1, ABCG1 expression levels were significantly upregulated in the HG-induced mTECs with TSF and SIS3 treatment. CONCLUSION: TSF can promote the cellular cholesterol efflux in HG-induced mTECs vis suppression of TGF-β1/Smad3 pathway.
