Jolkinolide B inhibits the proliferation and metastasis of colon cancer cells via blocking PI3K/Akt/NF-κB pathway
10.12092/j.issn.1009-2501.2021.03.002
- Author:
Linjun CHEN
1
;
Wenbin CHEN
1
;
Linjun CHEN
2
Author Information
1. Department of Surgery, The First Affiliated Hospital of Zhejiang University Medical College
2. Department of Surgery, Zhejiang Youth Hospital
- Publication Type:Journal Article
- Keywords:
EMT;
HT-29 cells;
Jolkinolide B;
Migration;
PI3K/Akt/NF-κB pathway;
Proliferation
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2021;26(3):250-257
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the inhibitory effect and mechanism of Jolkinolide B (JB) on the proliferation and metastasis of colon cancer HT-29 cells. METHODS: HT-29 cells were treated with different concentrations of JB, the cell proliferation rate was detected by MTT method, the cell clone formation rate was detected by plate cloning experiment, the cell cycle change was detected by flow cytometry, the cell migration ability was analyzed by wound healing assay, the cell invasion ability was studied by Transwell assay, E-cadherin, N-cadherin, vimentin, Snail1, Snail2, matrix metalloproteinase (MMP)-2 and MMP-9 protein expression levels were detected by immunofluorescence and Western blotting, and p-PI3K, PI3K, p-Akt, Akt, NF-κB P65and p-NF-κB P65 protein expression levels were detected by Western blotting. HT-29 cells were treated with 100 μg/L IGF-1 and 100 μg/L IGF-1+20 μmol/L JB, respectively. The expression of PI3K/Akt/NF-κB pathway related proteins was detected by Western blotting. RESULTS: JB inhibited the proliferation of HT-29 cells in a concentration-dependent manner, with an IC
