OCTN2 gene polymorphisms affect the chemotherapeutic sensitivity of oxaliplatin in colorectal cancer
10.12092/j.issn.1009-2501.2021.04.007
- Author:
Qiong LU
1
;
Haihong ZHU
1
;
Tingting QI
1
;
Guohua LI
1
;
Xinqi TENG
1
;
Jian QU
1
;
Qiang QU
2
Author Information
1. Department of Pharmacy, the Second Xiangya Hospital, Central South University, Institute of Clinical Pharmacy, Central South University
2. Department of Pharmacy, Xiangya Hospital, Central South University
- Publication Type:Journal Article
- Keywords:
Chemotherapeutic sensitivity;
Colorectal cancer;
OCTN2 transporter;
Oxaliplatin
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2021;26(4):401-407
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effect of OCTN2 gene polymorphism on the expression and function of OCTN2, as well as the sensitivity of SW480 cells to oxaliplatin. METHODS: Four mutations of OCTN2 (F17L, E317K, S467C and P478L) transfected cell lines were constructed. Real-time RT-PCR and Western blot were used to detect the levels of OCTN2 mRNA and protein. The content of oxaliplatin was detected by HPLC. MTS assay was used to detect cell viability. RESULTS: The expression level of all mutant OCTN2 mRNA and protein was not significantly different from that of wild-type OCTN2. Oxaliplatin uptake experiments showed that there was no significant difference in V
