Reversal effects and mechanisms of Flavonoids from Tetrastigma hemsleyanum on drug resistance in gefitinib-resistant lung cancer cells
10.12092/j.issn.1009-2501.2021.04.002
- Author:
Jiaqi HE
1
;
Juanjuan LI
1
;
Xiaoai LV
1
;
Huanhuan ZHANG
2
;
Chenhuan YU
2
;
Chenhuan YU
3
Author Information
1. The First Affiliated Hospital of Zhejiang Chinese Medical University
2. Laboratory Animal Center, Hangzhou Medical Collage
3. Institute of Cancer and Basic Medicine, Chinese Academy of Sciences
- Publication Type:Journal Article
- Keywords:
EGFR-TKIs;
Flavonoids;
PTEN/PI3K/AKT;
Resistance;
Tetrastigma hemsleyanum Radix
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2021;26(4):368-375
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the sensitization of flavonoids from Tetrastigma hemsleyanum (FTH) on gefitinib (GEF)-resistant lung adenocarcinoma cells. METHODS: The viabilities of A549 and A549/GR cells treated with FTH and GEF were detected by MTT method. The apoptotic rates and cell cycles of A549/GR cells treated with FTH and GEF were detected by Flow cytometry. The anti-tumor effects of flavonoids from FTH and GEF were assayed in A549/GR tumor-bearing mice. The expressions of proteins (PTEN, PI3K, p-PI3K, AKT, p-AKT) were detected by Western blot analysis. RESULTS: Compared with GEF group, FTH significantly enhanced the inhibition of GEF on the proliferation of A549/GR cells (P< 0.05). Combination with FTH and GEF significantly increased the apoptosis of A549/GR cells which were arrested at the G
