Reversal of 5-fluorouracil resistance in hepatocellular carcinoma cells by inhibiting ribonucleotide reductase M2
10.12092/j.issn.1009-2501.2021.07.002
- Author:
Saihong YU
1
;
Xiaoliang ZHENG
1
;
Yiyi PU
1
;
Dongmei YAN
1
;
Xiaoju WANG
1
;
Jie YU
1
;
Saihong YU
2
;
Jie YU
2
Author Information
1. School of Bioengineering, Hangzhou Medical College
2. Zhejiang Provincial Key Laboratory of Neuropsychiatric Drug Research, Hangzhou Medical College
- Publication Type:Journal Article
- Keywords:
3-AP(Triapine);
5-fluorouracil;
Hepatocellular carcinoma;
Resistance;
Ribonucleotide reductase M2
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2021;26(7):729-737
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the role of ribonucleotide reductase M2 (RRM2) in 5-fluorouracil (5-FU) resistance of HCC cells and to develop potential strategies to enhance the sensitivity of HCC cells to 5-FU. METHODS: The expression of RRM2 was examined by Western blot in BEL/5-FU cells and BEL7402 cells. The expression of RRM2 was down-regulated through RNA interference (RNAi) technology and the activity of RRM2 was inhibited by the RRM2 inhibitor 3-AP. The cell proliferation ability was detected by CCK-8 assay and colony formation assay, and the apoptosis was analyzed by Confocal high-content system. RESULTS: The expression level of RRM2 was increased by 2.5-fold in BEL/5-FU cells compared with BEL7402 cells. Compared with control siRNA, the half maximum inhibitory concentration IC
