Tangshen formula improves cholesterol uptake and efflux of macrophages induced by high lipid via activating PGC-1α/LXR/ABCA1 pathway
10.12092/j.issn.1009-2501.2021.09.002
- Author:
Ke XU
1
;
Junwei GAO
1
;
Chenguang WU
1
;
Feng TIAN
1
;
Lifan WANG
1
;
Peng LIU
2
;
Zhengri SHEN
2
;
Hui JIA
2
;
Ping LI
3
Author Information
1. Heilongjiang Academy of Chinese Medicine Sciences
2. Shunyi Hospital, Beijing Traditional Chinese Medicine Hospital
3. Beijing Key Lab Immune-mediated Inflammatory Diseases, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital
- Publication Type:Journal Article
- Keywords:
CD36;
Hyperlipidemia;
PGC-1α/LXR/ABCA1 pathway;
Tangshen formula
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2021;26(9):978-985
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the effects of Tangshen formula (TSF) treatment on lipid efflux and uptake in sodium palmitate (PA) induced RAW264.7 macrophages. METHODS: After 200 μmol/L PA induced RAW264.7 macrophages, TSF and PGC-1α-siRNA were given to intervene respectively. The lipid content in the cells was detected by ELISA kit; intracellular lipid droplet deposition was detected by BODIPY 493/503 and Filipin staining. Western blot and Real-time PCR were used to detect the expression of PGC-1α, LXR, ABCA1 and CD36. RESULTS: TSF diminished the levels of TC, TG and intracellular lipid droplet deposition in PA-induced RAW264.7 macrophages. Western blot and Real-time PCR analysis showed that TSF could up-regulate the expression of PGC-1α, LXR, ABCA1 and down-regulate the expression of CD36. Furthermore, silencing PCG-1α by SiRNA significantly suppressed the effects of upregulating the expression of PGC-1α, LXR and ABCA1, and downregulating the CD36 expression with TSF treatment. CONCLUSION: TSF may extenuate intracellular lipid droplet deposition in macrophages by upregulating cholesterol efflux through activating the PGC-1α/LXR/ABCA1 pathway and inhibiting lipid uptake through down-regulateing the expression of CD36.
