Effects of oxypeucedanin on the resistance of breast cancer MCF-7/DOX cells to doxorubicin
10.12092/j.issn.1009-2501.2022.03.003
- Author:
Wei DONG
1
;
Xiaoying HUANG
1
;
Guowei ZHAO
1
;
Xinli LIANG
1
;
Bingbin XIE
2
;
Xilan TANG
3
;
Hongming LI
3
;
Yumei QIU
3
Author Information
1. Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Traditional Chinese Medicine
2. Department of Otorhinolaryngology Head and Neck Surgery, the Second Affiliated Hospital of Nanchang University
3. School of Pharmacy, Jiangxi Science & Technology Normal University
- Publication Type:Journal Article
- Keywords:
Breast cancer;
Doxorubicin;
Drug resistance;
Glycerophospholipid metabolism;
Oxypeucedanin
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2022;27(3):260-266
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of oxypeucedanin (OPD) on doxorubicin resistance in human breast cancer MCF-7/DOX cells and its possible mechanism. METHODS: MCF-7/DOX cells were cultured in vitro, MTT assay was used to detect the effect of OPD on the survival of MCF-7/DOX cells, and the effect of OPD combined with different concentrations of doxorubicin on the proliferation of MCF-7/DOX cells were investigated. The effect of OPD combined with doxorubicin on the expression of genes including MDR1, MRP1, AGPAT2, CHKA, CEPT1, DGKA, PCYT1A, PLA2G15 in MCF-7/DOX cells was measured by qRT-PCR. The effect of OPD combined with doxorubicin on the protein expression of MDR1, MRP1, CHKA and CCTα in MCF-7/DOX cells was determined by Western blot. RESULTS: The IC
