Effect of Qiteng Xiaozhuo granule mediating miR-339-5p on inflammatory indexes in rats with chronic glomerulonephritis
10.12092/j.issn.1009-2501.2022.04.017
- Author:
Jiarong GAO
1
;
Miaomiao SHI
1
;
Hao CHEN
1
;
Hui JIANG
1
;
Xiujuan QIN
1
;
Miaomiao SHI
2
;
Jiarong GAO
3
;
Hao CHEN
3
;
Hui JIANG
3
Author Information
1. Department of Pharmacy, The First Affiliated Hospital of Anhui University of Chinese Medicine
2. College of Pharmacy, Anhui University of Chinese Medicine
3. Anhui Province Key Laboratory of Chinese Medicinal Formula
- Publication Type:Journal Article
- Keywords:
Chronic glomerulonephritis;
Inflammatory cytokines;
MiR-339-5p;
Qiteng Xiaozhuo granule
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2022;27(4):457-465
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of Qiteng Xiaozhuo granule on inflammation indexes in rats with chronic glomerulonephritis (CGN) through miR-339-5p. METHODS: CGN model was established by adriamycin (ADR) injection into tail vein of rats. Qiteng Xiaozhu granules (21.6, 10.8, 5.4 g/kg) of different doses were intragastric-fed for 30 days. Pathological changes in kidney tissues of CGN rats were observed by HE staining and electron microscopy. The contents of IL-1β, IL-6, IL-10 and TNF-α in serum and kidney tissue of CGN rats were detected by qRT-PCR, ELISA and Western blot. Relative expressions of miR-339-5p, Syk and p-Syk proteins were detected by Western blot, the relative expressions of miR-339-5p mRNA and Syk mRNA were detected by qRT-PCR. RESULTS: There were thickening of basement membrane and glomerular atrophy in the model group. Compared with the normal group, the expression of miR-339-5p and IL-10 in the model group was significantly down-regulated, while the expression of IL-1β, IL-6, TNF-α and Syk was significantly up-regulated. Qiteng Xiaozhuo granule group could significantly reduce the protein and mRNA expression levels of IL-1β, IL-6, TNF-α, Syk in kidney tissue.CONCLUSION: Qiteng Xiaozhuo granule may down-regulate the expression of inflammatory factors through miR-339-5p in the treatment of inflammatory symptoms in CGN rats.
