Study on the treatment of thin endometrium with growth hormone and vitamin E
10.12092/j.issn.1009-2501.2022.08.003
- Author:
Jiamin DING
1
;
Xiali ZHAO
1
;
Genfeng JIANG
1
;
Siwen CHEN
1
;
Chengcheng HONG
1
;
Weihua HU
1
;
Youdi WANG
2
;
Shuqin LI
3
Author Information
1. Department of Reproductive Medicine Center, The First Affiliated Hospital of Wannan Medical College
2. Department of Tissue and Embryology, Wannan Medical College
3. Department of Obstetrics and Gynecology, The First Affiliated Hospital of Wannan Medical College
- Publication Type:Journal Article
- Keywords:
cell proliferation;
growth hormone;
thin endometrium;
vitamin E
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2022;27(8):857-862
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of growth hormone (GH) and vitamin E (Vit.E) combined in the treatment of endometrial thinning. METHODS: Twenty female SD rats were randomly divided into four groups: control group, model group, GH group and treatment group, with 5 rats in each group. Control group was routinely fed; Rats in model group, GH group and treatment group were injected intrauterine with 95%ethanol during estrus stage to construct a thin endometrial model. Six to eight hours after operation, rats in model group were injected intrauterine with 0.2 mL normal saline, rats in GH group and treatment group were injected with the same amount of GH, and the treatment group was given intragastric treatment of 60 mg/kg Vit.E. The rats were sacrificed 3 estrus cycles (about 2 weeks) after the operation. HE staining was performed on the uterine tissue to identify the model, and the levels of Cytokeratin 19 and Vimentin in the endometrium were detected by immunohistochemical color. RESULTS: The endometrial thickness of the model group was significantly thinner than that of the model group, and the endometrial thickness of the treatment group was significantly higher than that of the control group, but the endometrial thickness of the GH group was slightly lower than that of the control group. The expression levels of keratin and vimentin in model group were lower than those in GH group, control group and treatment group, and the differences were statistically significant. CONCLUSION: Endometrial-related proliferation indexes were significantly increased after GH and vitamin E treatment, and GH and vitamin E could effectively promote the proliferation of endometrial cells.
