Tyrosine phosphorylation of TRPM3 ion channel mediates diabetes-induced heat hyperalgesia
10.12092/j.issn.1009-2501.2022.09.002
- Author:
Li YANG
1
;
Shasha HE
1
;
Yue JIN
1
;
Chengsong LIU
1
;
Huhu BAI
2
Author Information
1. Department of Pharmacy, Affiliated Hospital of Gansu University of Chinese Medicine
2. School of Life Science, Lanzhou University
- Publication Type:Journal Article
- Keywords:
allodynia;
diabetic peripheral neuropathy;
DRG;
hyperalgesia;
TRPM3;
tyrosine phosphorylation
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2022;27(9):971-976
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the relationship between TRPM3 and diabetes-induced painful peripheral neuropathy. METHODS: Treptozotocin (STZ) was intraperitoneal injected for establishment of diabetic mice model, behavioral tests of paw withdraw thresholds (PWTs) and paw withdraw latencies (PWLs) were conducted; Protein contents and tyrosine phosphorylation levels of TRPM3 were detected by immunoprecipitation and immunoblotting. RESULTS: The PWTs and PWLs in diabetic mice were significantly reduced; TRPM3 tyrosine phosphorylation in the dorsal root ganglia (DRG) of diabetic mice significantly increased compared with control, while the protein expression shows no statistical significance; Enhanced tyrosine phosphorylation of TRPM3 by BPV can evoke heat hyperalgesia in intact mice; Reduce of the tyrosine phosphorylation levels of TRPM3 through PP2 significantly alleviates diabetes-induced heat hyperalgesia, without affecting mechanical allodynia. CONCLUSION: The upregulation of tyrosine phosphorylation of TRPM3 plays a key role in heat related painful diabetic peripheral neuropathy.
