Dioscin attenuates inflammatory injury in uric acid-induced renal tubular epithelial cells by suppression of NF-κB signaling pathway
10.12092/j.issn.1009-2501.2022.10.003
- Author:
Peng LIU
1
;
Chen WANG
1
;
Yun WANG
1
;
Xinping QIU
1
Author Information
1. Shunyi Hospital, Beijing Traditional Chinese Medicine Hospital
- Publication Type:Journal Article
- Keywords:
Dioscin;
hyperuricemic nephropathy;
inflammatory response;
NF-κB P65 inhibitor BAY11-7082;
NF-κB signaling pathway
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2022;27(10):1099-1105
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the effect of Dioscin treatment on NF-κB signaling pathway and cellular inflammatory injury and explore its potential mechanism in uric acid-induced mouse tubular epithelial cells (mTECs). METHODS: After 1.2 mol/L uric acid induced mTECs, Dioscin and NF-κB P65 inhibitor BAY11-7082 were given to intervene respectively. IκB-α, NF-κB P65, PP65, NLRP3, IL-1β and β-actin were detected by Western Blot, immunofluorescence staining and real-time PCR. RESULTS: Western Blot, immunofluorescence staining and real-time PCR analysis showed that expression levels of PP65, NLRP3 and IL-1β were significantly downregulated in the uric acid-induced mTECs with Dioscin and BAY11-7082 treatment. CONCLUSION: Dioscin attenuates uric acid-induced cellular inflammatory damage by suppression NF-κB signaling pathway.
