Cordyceps sinensis extracts attenuates HBx-induced mesangial cell proliferation and extracellular matrix synthesis by suppression of the PI3K/Akt pathways
10.12092/j.issn.1009-2501.2022.11.006
- Author:
Jing LEI
1
;
Ping HE
1
;
Wenlin CAI
1
;
Yongzhe ZHANG
1
;
Beiru ZHANG
1
;
Dajun LIU
1
Author Information
1. Department of Nephrology, Shengjing Hospital of China Medical University
- Publication Type:Journal Article
- Keywords:
cordyceps sinensis;
HBx;
human mesangial cells;
matrix accumulation;
PI3K/Akt;
proliferation
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2022;27(11):1247-1254
- CountryChina
- Language:Chinese
-
Abstract:
AlM: To investigate the effect of C. sinensis extracts on HBx (Hepatitis B virus X protein) induced cell proliferation and extracellular matrix (ECM) accumulation and the underlying mechanism in cultured human mesangial cells (HMCs). METHODS: Human mesangial cells were stable transfected with pCMV-HBx to establish an HBx over-expression model, and a control group was transfected with an empty vector. Cell proliferation was determined by MTT assay and DNA synthesis assay. Western blotting was used to measure the expression of PI3K/Akt signaling pathway-related proteins and extracellular matrix. RESULTS: HBx transfection induced cell proliferation, matrix accumulation. HBx-transfected mesangial cells had increased activity of the PI3K/Akt pathways, and treatment with C. sinensis suppressed this effect. CONCLUSlON: C.sinensis attenuates the HBx-induced human mesangial cell proliferation and matrix production in human mesangial cells via inhibition of the PI3K/Akt pathways.
