Interventional effect of Radix Angelica Sinensis and Radix Hedysari ultrafiltration on miR-21-5P targeting TGF-β1-mediated radiation-induced myocardial fibrosis
10.12092/j.issn.1009-2501.2022.11.003
- Author:
Chengwen JIA
1
;
Hugang JIANG
1
;
Jinyan HAN
1
;
Xiang GAO
2
;
Xiaodong XU
2
;
Xinke ZHAO
2
Author Information
1. Gansu University of Traditional Chinese Medicine
2. The Affiliated Hospital of Gansu University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
miR-21-5P;
radiation-induced myocardial fibrosis;
Radix Angelica Sinensis and Radix Hedysari ultrafiltration;
TGF-β1
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2022;27(11):1221-1230
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To reveal that the targeted regulation of TGF-β1 by miR-21-5P is the key mechanism that mediates the activation of myocardial fibroblasts, and to clarify the intervention ofRadix Angelica Sinensis and Radix Hedysari ultrafiltration on the mechanism of miR-21-5P targeting to regulate TGF-β1 effect. METHODS: (1) The cells were randomly divided into normal group and irradiation group. The irradiation group received 6Gry single irradiation, and then RT-PCR was used to detect miR-21-5P, and Western Blot was used to detect the expression of α-SMA and TGF-β1. (2) The cells were randomly divided into normal group, irradiation group, miR-21-5P
