Comparative study on the ability of active ingredients in Jinfukang to decrease neutrophils recruiting by lung cancer circulating tumor cells
10.12092/j.issn.1009-2501.2023.01.001
- Author:
Jiajun LIU
1
;
Zujun QUE
1
;
Jianhui TIAN
1
;
Jianhui TIAN
2
Author Information
1. Oncology Research Center, Hospital of Traditional Chinese Medicine Affiliated to Shanghai University of Traditional Chinese Medicine
2. Oncology Clinical Research Center, Hospital of Traditional Chinese Medicine Affiliated to Shanghai University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
circulating tumor cells;
jinfukang;
lung cancer metastasis;
neutrophils;
trigonelline
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2023;28(1):1-9
- CountryChina
- Language:Chinese
-
Abstract:
AiM: To observe the recruitment effect of lung cancer circulating tumor cells (CTCs) on neutrophils, and find out the effective components of jinfukang in inhibiting the recruitment of neutrophils by CTCs. METHODS: The ability of human lung adenocarcinoma circulating tumor cells CTC-TjH-01 cells to recruit neutrophils from whole blood leukocytes, and the effect of jinfukang and its six active ingredients on the recruitment of neutrophils by CTCs was detected by flow cytometry. CCK-8 assay was used to observe cell viability to determine the concentration of action; transwell chemotaxis assay was used to detect the effect of six active ingredients on the chemotaxis of CTC-TjH-01 cells to neutrophils. RESULTS: CTCTjH-01 cells could increase the recruitment of neutrophils compared to blank control (p < 0.01); after the effect of jinfukang, the neutrophils recruited by CTC-TjH-01 cells decreased (p < 0.01). Trigonelline and Ophiopogonin W reduced neutrophil recruitment to CTC-TjH-01 cells at concentrations that had no effect on cell viability (p < 0.01), trigonelline had the best effect; the chemotaxis of CTC-TjH-01 cells to neutrophils was weakened by trigonelline, astragaloside IVz and Ophiopogon pol-ysaccharide (p < 0.05), and trigonelline had the best effect. CONCLUSiON: jinfukang can inhibit the recruitment of neutrophils by circulating tumor cells, and trigonelline, an effective monomer with "Fuzheng" effect in jinfukang, can significantly inhibit the recruitment of neutrophils by circulating tumor cells in lung cancer, which proves that trigonelline may have the potential to inhibit lung cancer metastasis through targeting neutrophils.
