Sequoiaflavone inhibits stem cell properties such as proliferation and invasion of gastric cancer cells by down-regulating PI3K/AKT signaling pathway
10.12092/j.issn.1009-2501.2023.05.004
- Author:
Yan WANG
1
;
Jinhui ZHANG
1
;
Yongchen ZHAO
1
;
Hongxiang LIU
1
;
Yawei LIU
1
;
Huanhuan MIAO
1
;
Xincai YANG
1
Author Information
1. Department of Integrative Chinese and Western Medicine, Affiliated Hospital of Hebei University
- Publication Type:Journal Article
- Keywords:
AKT;
gastric cancer;
invasion;
migration;
PI3K;
proliferation;
sequoiaflavone
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2023;28(5):508-513
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the molecular mechanism of sequoiaflavone affecting gastric cancer cells. MEHTODS: Gastric cancer cell line AGS cells were treated with gradient concentrations of sequoiaflavone, and then induced by PI3K/AKT signaling pathway activator. The optimal inhibitory concentration and time of semi-inhibitory concentration of red cedar flavonoid on AGS cells were detected by CCK-8, and the changes of cell proliferation, migration and invasion ability were detected by colony formation assay, transwell assay and wound healing assay. PI3K/AKT signal pathway related proteins p-PI3K, PI3K, p-AKT and AKT were detected by western blot. RESULTS: Sequoiaflavone inhibited AGS cells in a concentration-dependent manner. The half inhibitory concentration was 0.5 mmol/L, the optimal treatment time was 48 h. The protein expression of p-PI3K and p-AKT was down regulated. The proliferation, migration and invasion of AGS cells were decreased after treated with sequoiaflavone. After treated with PI3K / AKT signal pathway activator, the protein expression level of pPI3K and p-AKT was partially reversed, and the ability of cell viability, proliferation, migration and invasion was also partially improved. CONCLUSION: Inactivation of PI3K/AKT signaling pathway caused by sequoiaflavone inhibited gastric cancer cells proliferation, migration and invasion ability.
