Effects of geniposide metabolites genipin on induced HK-2 cells injury and NLRP3 pathway
10.12092/j.issn.1009-2501.2023.05.001
- Author:
Mingzhu SHI
1
;
Tianxiang YE
1
;
Yixuan LIU
1
;
Huifang LI
1
Author Information
1. College of Traditional Chinese Medicine and Food Engineering, Shanxi University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
genipin;
HK-2 cell;
NLRP3;
toxicity
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2023;28(5):481-488
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the toxicity of genipin-a kind of geniposide metabolites induced human tubular epithelial cells HK-2 and its effect on NLRP3 pathway. METHODS: The dose of GP on HK-2 cells were preliminarily determined by CCK8 method, the apoptosis or necrosis rate of HK-2 cells was detected by Hoechst 33342 / PI, the level of LDH release and reactive oxygen species was detected by Kits, and mitochondrial membrane potential and intracellular calcium ion concentration were detected by high content imaging. Real-time PCR detected mRNA levels of kindey injury factor-1, osteopontin, NLRP3, Caspase-1, interleukin 1β, and interleukin 18. RESULTS: Compared with the 0 μg / mL group, GP>50 μg/mL significantly reduced cell viability (P< 0.05, P<0.01), and the IC50 value was 110.50 μg/mL. Set the control group, the low, medium and high dose groups of GP (50, 100, 200 μg/mL); Compared with the control group, the cell density decreased in the medium and high dose groups of GP, and the PI positivity, LDH release, ROS, Ca
